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CAS Number: 9001-53-0
MDL: MFCD00130946
Storage Temperature: -20 °C
TEST Specification
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units solid > 0.05U/mg
Enzymatic Activity
One unit will oxidize 1.0 micromole of Putrescine per hour at pH 7.2 at 37 deg C.
Impurity < 1 % _
(Monoamine Oxidase)

(EC) Number 1.4.3.6

性質
Related Categories 1.4.x.x Acting on CH-NH2, 1.x.x.x Oxidoreductases, Enzyme Class Index, General Metabolic Enzymes, General Metabolic Enzymes A-H,
solubility   100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL
foreign activity   monoamine oxidase (benzylamine substrate) ≤1%
storage temp.   ?20°C
產品描述
二胺氧化酶包裝
1, 5, 10 g in poly bottle
250 mg in poly bottle
Unit Definition
One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.
Application
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.
Biochem/physiol Actions
Diamine oxidase from porcine kidney is a homodimer consisting of 2 equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.[1] The molecular mass of the enzyme is found to be 170 kDa.[2] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[3] It catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.[1] The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. It is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.